Genome Sequencing &
Analysis Core Resource -- January
2013
Welcome
to our Update and Message
Center.
You are
receiving this newsletter because you have
recently used the Duke IGSP Genome Sequencing
& Analysis Core Resource sequencing
services. This newsletter will allow you to
follow recent developments with regards to
instrument status, new instruments and
services, changes in policies, and other
happenings in the IGSP Genome Sequencing &
Analysis Core Resource. We encourage you to
scan the headlines and read any topics that
relate to your research. We will endeavor to
keep each item brief and to the point. We will
send out these newsletters each month by email.
See footer of this email to unsubscribe and to
access archives.
New
Instruments
Ion Proton
Arrival. We have just taken
delivery of the new Ion Proton. It
should be available for service
sometime in February. Existing
protocols for the Ion Torrent PGM
should be easy to move to the Proton
for larger scale reads. http://tinyurl.com/7y7thdq
Illumina HiSeq
Upgrade. This upgrade to the
Illumina HiSeq (HiSeq 2500) enables the
use of a “Rapid Run” (of about 27
hours) which is much shorter than the
current lengthy 11 day current run
time. It will generate at least 200
million single reads per flowcell (1
lane), with a maximum read length of
150 bp. http://tinyurl.com/ao379j3
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New
Products and Services
SureSelect Exome
Capture is now offered and we
will soon offer Haloplex service. http://tinyurl.com/8ah8o4u
New PacBio SMRT cells
(v.3) are optimized for better
loading, allowing to sequence more SMRT
cells from one library.
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Operation Updates
New Schedule For
Sequencing Fragment Analysis.
Fragment analysis will now be performed
one day per week on Thursdays. Samples
must be received by 5pm on Wednesdays,
and data will be available on Friday
morning.
Sample
Retention. Effective
immediately, unless otherwise requested
all samples and libraries will be
discarded two months after sequencing
data has been released.
Reminder about Data
Storage. If you need storage
for your sequencing data or
computational infrastructure there are
options available within IGSP. For
further information visit the sites: http://www.genome.duke.edu/cores/computational/services/storage/
and http://www.genome.duke.edu/cores/computational/services/computation/
Unfortunately, we do not have
sufficient capacity to keep your data
on our servers longer than 4
weeks.
Sample Submission
Reminder. Please remember to
bring your sample submission form and a
copy of your order. These documents are
necessary for us to track samples, and
without them there is a risk that your
samples will be misidentified or
lost.
Any
Questions? Send an email to consult-seqcore@duke.edu
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Project Highlight
One of our latest PacBio runs
reached a new record by producing more
than 30,000 sequences per SMRT cell at
~8kb average read length. (see read
length plot below) .
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